CBSE Biotechnology
Class 12 Syllabus
Exam
Structure
|
Units
|
Topics
|
Marks
|
|
Unit V
|
Protein and Gene Manipulation
|
40
|
|
Unit VI
|
Cell Culture and Genetic
Manipulation
|
30
|
|
|
Practicals
|
30
|
|
|
Total
|
100
|
Unit
V: Protein and Gene Manipulation
Chapter-1: Recombinant DNA
Technology
Introduction; Tool
of DNA Technology; Marketing Recombinant DNA; Introduction of
Recombinant DNA into Host Cells; Identification of Recombinants; DNA
Library; DNA Probes; Hybridization Techniques; Polymerase Chain
Reaction (PCR); DNA Secquencing; Site-directed Mutagenesis
Chapter-2:
Protein Structure and Engineering
Introduction
to the World of Proteins; 3-D Shape of Proteins; Structure-Function
Relationship in Proteins Purification of Proteins; Characterization
of Proteins; Protein based Products; Designing Proteins (protein
engineering)
Chapter-3: Genomics and
Bioinformatics
Introduction; Genome
Sequencing Projects; Gene Prediction and Counting; Genome Similarity,
SNPs and Comparative Genomics; Functional
Genomics; Proteomics; History of Bioinformatics; Sequences and
Nomenclature; Information Sources; Analysis using Bioinformatics
Tools
Unit VI: Cell Culture and Genetic Manipulation
Chapter-1:
Microbial Culture and Applications
Introduction,
Microbial culture techniques, Measurement and kinetics of microbial growth,
Scale up of microbial process, Isolation of microbial products, Strain
isolation and improvement, Applications of microbial culture technology,
Biosafety issues in microbial technology
Chapter-2: Plant Cell Culture and
Applications
Introduction; Cell
and Tissue Culture Techniques; Applications of Cell and Tissue
Culture; Gene Transfer Methods in Plants; Transgenic Plants with
Beneficial Traits; Biosafety in Plant Genetic Engineering
Chapter-3:
Animal Cell Culture and Applications
Introduction,
Animal cell culture techniques, Characterisation of cell lines, Methods of gene
delivery into cells, Scale-up of animal culture process, Applications of animal
cell culture, Stem cell technology, Tissue engineering
Practicals
- Use of special equipment in biotechnology experiments.
- Isolation of bacterial plasmid DNA
- Detection of DNA by gel electrophoreses
- Isoloation of Genomic DNA (CTAB method)
- Estimation of DNA
- Bacterial transformation using any plasmid
- Restriction digestion of plasmid DNA & its analysis by gel electrophoreses
- Isolation of bacterial grom curd & staining of bacteria
- Cell viability assay
- Data retrieval and data base search using internet site NCBI and download a DNA and protein sequence from internet, analyse it and comment on it
- Reading of a DNA sequencing gel to arrive at the sequence
- Project work
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